Journal: bioRxiv
Article Title: JAK/STAT pathway promotes Drosophila neuroblast proliferation via the direct CycE regulation
doi: 10.1101/2020.07.10.195875
Figure Lengend Snippet: (A , B) Representative confocal images of wing discs showing CycE(Stat92E-WT)-lacZ or CycE(Stat92E-MT)-lacZ expression (green, β-galactosidase antibody staining) in the en-Gal4,UAS-Stat92E ΔNΔC , UAS-RFP (red) genetic background. DAPI (blue) is used to label nuclei. Arrows, induced CycE(Stat92E-WT)-lacZ expression in the en -expressing region of the wing disc. (C , D) Representative confocal images showing cell body region of MB complex at pupal stages. CycE or CycE(Stat92E-WT)-lacZ expression (red, CycE or β-galactosidase antibody staining) was shown in the wild type MB labeled by GAL4-OK107>UAS-mCD8-GFP (green). Arrows, MB Nbs labeled with CycE or CycE(Stat92E-WT)-lacZ . (E) CycE (red, CycE antibody staining) and Dpn (green, Dpn antibody staining) levels in the Stat92E ts ( Stat92EF/Stat92E 06346 ) MBs at the first day pupal stage. Temperature and days after switch are shown. Arrows, Nbs co-stained with Dpn and CycE. (F) Box plots comparing the CycE levels in MB Nbs of the first-day pupa brain from flies always incubated at 25°C (n = 32 Nbs from 8 biological samples) and flies switched to 29°C (n = 16 Nbs from 4 biological samples) for 2 days. Two-tailed t-test was used to calculate the statistical significance. Scale bar, 25 μm.
Article Snippet: The following primary antibodies were used: rat anti-Dpn antibody (a gift from Chris Doe) (1: 10); rabbit anti-β-Galactosidase antibody (1: 50); and rabbit anti-CycE antibody (Santa Cruz Biotechnology) (1:50).
Techniques: Expressing, Staining, Labeling, Incubation, Two Tailed Test